A simple, accurate, rapid and precise isocratic reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of clopidogrel bisulphate in tablet. The chromatographic separation was carried out on an Inertial BDS C18 250 x 4.6 x 5 mm column with a mixture of Methanol: Water (70:30) as mobile phase; at a flow rate of 1 ml/min. UV detection was performed at 222 nm. The retention times of clopidogrel bisulphate are 3.45. Calibration plots were linear (r 2>0.994) over the concentration range 2-10 μg/ml for clopidogrel bisulphate. The method was validated for accuracy, precision, linearity, and LOD & LOQ. The proposed method was successfully used for quantitative analysis of tablet. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of clopidogrel bisulphate in bulk drug and tablet dosage form.
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